HPLC/MS/MS QUANTITATION OF PACLITAXEL IN BLOOD AND TISSUES
Taxol (paclitaxel, PTX) is a very useful, but toxic, agent used to inhibit cellular growth. Therefore, it has been used to treat cancers, rheumatoid arthritis, and arterial restenosis following stent placement. Due to the inherent toxicity of PTX, it is important to measure the concentration is blood and tissues during delivery system development.
PTX is measured in drug formulations using HPLC with UV detection. However, PTX in blood and solid tissues must be measured using HPLC/MS/MS so that there will be adequate sensitivity. Our HPLC/MS/MS assay is fully validated for both serum and solid tissues.
The method developed and validated at RML is able to separate PTX from it's epimer, 7-epi-paclitaxel, and other taxanes. The LOD is 100pg/mL and the LOQ is 150pg/mL. We are quite experienced in overcoming the problems associated with the analysis of PTX and other drugs in tissues. We are cGLP compliant and FDA inspected. We also are CLIA licensed. We offer clinical and research assays for other anti-proliferative agents, such as everolimus, sirolimus, cyclosporine, mycophenolic acid, and tacrolimus.
One of our specialties is the development of new drug assays for the pharmaceutical industry.
For additional information, please contact either Dr. Patricia Sulik or Dr. Robert Lantz.
Mountain Instrumental Laboratories, Inc.
108 Coronado Ct.
Ft. Collins, CO 80525
LAST MODIFIED: 17 APRIL 2012