FIPRONIL and Ivermectin are important and relatively new insecticides for crop and animal protection. They can be quite effective.  However, as with all pesticides, they is not without toxicity to unintended organisms (1, 2).  The metabolism, degradation, and environmental fate have been studied (3, 4, 6).  Studies on toxicity are continuing, as are new applications and application methods (7).  The major fipronil metabolites are fipronil-sulfone and fipronil-sulfide.  A major photodegradation product is fipronil-desulfinyl, which can be ingested by animals and also appears following oral exposure or injection.  All three products appear to contribute to the toxicity of fipronil.  Therefore, analyses should include all four substances. There also is a fipronil-amide hydrolysis product whose analysis we can provide. 

We use HPLC/MS/MS (tandem MS) for each of these and other agents (5), which provides better sensitivity than does GC/MS (5).  However, if GC/MS analysis is required, we can offer this, too.  Our limits of detection for routine analysis of Ivermectin, fipronil, and metabolites is 400pg/mL with a 1mL sample.  The lower limit of quantitation for routine samples is generally 500pg/mL with a 1mL sample.  

We also analyze biological samples for similar pesticides, such as emamectin and selamectin and other endectocides (8).   There are two major groups of macrolide endectocides, the avermectin (invermectin, avermectin, selamectin, doramectin, inter alia) and the milbemycins (moxedectin is the most common). 

Please contact us for costs and turn around time.  Ask for Dr. Patricia Sulik or Dr. Robert Lantz. 



1.    "FIPORONIL Technical Fact Sheet" National Pesticide Information Center.

2.    "New Pesticide Fact Sheet - Fipronil" EPA- 737-F-96-005 USEPA, Office of Pesticide Programs (1996).

3.    Hainzl, D, Cole, LM and Casida, JE, "Mechanism for Selective Toxicity of Fipronil Insecticide and Its Sulfone Metabolite and Desulfinyl Photoproduct," Chem. Res. Tox. 11 1529-1535 (1998).

4.    Hainzl, D and Casida, JE, "Fipronil Insecticide:  Novel Photochemical desulfinylation with Retention of Neuotoxicity," PNASUS 93, 12764-12767 (1996).

5.    Pirard, C, et al, "Development and Evaluation of a Multi-residue method for pesticide Determination..." J. Chrom. A 1152 116-123 (2007).

6.    Bobe, A, et al, "Kinetics and Mechaminsms of Abiotic Degradation of Fipronil (Hydrolysis and Photolysis)," J. Agric. Food Chem. 46, 2834-2839 (1998).

7.    Sackmann, P, Rabinovich, M, and Corley, JC  "Successful Removal of German Yellow jackets (Hymenoptera Vespidae) by Toxic Baiting," J. Econ. Entomology 94 (4) 811-816 (2001).

8.    Rudik, I. et al, "Isolation and multiresidue detection of macrolide endectocides," J. Vet. Diagnostic Invest. 14, 295-302 (2002).


Rocky Mountain Instrumental Laboratories, Inc.
108 Coronado Ct.
Ft. Collins, CO 80525



Last Update:  17 APRIL 2012